Tissue Culture of the Enteric Nervous System from Equine Ileum

被引：0
|
作者：
N.P.H. Hudson
G.T. Pearson
I.G. Mayhew
机构：
[1] University of Edinburgh,Departments of Preclinical Veterinary Sciences and Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies
[2] University of Edinburgh,Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies
[3] University of Edinburgh,Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies
来源：
Veterinary Research Communications | 2000年 / 24卷
关键词：
culture; enteric nervous system; glia; horse; ileum; immunohistochemistry; neuron; submucous plexus;
D O I：
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摘要：
Ileal samples were harvested fresh from euthanized adult horses. The tissues were microdissected to prepare wholemount preparations for immunohistochemistry and for either explant or dissociated culture systems of the enteric nervous system. Explant culture systems were established using wholemounts of either the submucous plexus or the muscularis externa (including the myenteric plexus). Dissociated cell cultures could only be obtained from the submucous plexus. Culture systems were maintained for up to 5 days. Immunoreactivity for a neuronal marker (Pan-N) and for glial cell markers (GFAP and S100) indicated the presence of both neurons and enteric glia in the tissue culture preparations.
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页码：299 / 307
页数：8
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