Enzymes immobilized on montmorillonite: comparison of performance in batch and packed-bed reactors

被引：0
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作者：
Sanjay Gopinath
Sankaran Sugunan
机构：
[1] Department of Applied Chemistry,
[2] Cochin University of Science and Technology,undefined
[3] Department of Applied Chemistry,undefined
[4] Cochin University of Science and Technology,undefined
来源：
Reaction Kinetics and Catalysis Letters | 2006年 / 88卷
关键词：
adsorption; packed-bed reactor; batch reactor; covalent binding; Immobilization;
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学科分类号：
摘要：
The enzymes a-amylase, invertase and glucoamylase were immobilized on acid activated montmorillonite using two techniques, viz. adsorption and covalent binding, and their activities were tested in a batch and packed-bed reactor and were compared. The packed-bed reactor showed an improved performance for all immobilized enzymes, which was attributed to lowering of diffusional restrictions to mass transfer. Lower activity in case of batch reactor for immobilized invertase was due to a combined effect of loss of native conformation of enzyme on account of immobilization and mass transfer resistances due to improper diffusion of substrate to the active site of enzyme. For immobilized glucoamylase, the packed-bed reactor demonstrated exceptionally high activity that was very close to the free enzyme. Covalently bound glucoamylase showed higher activity than the free enzyme.
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页码：3 / 9
页数：6
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