Stem Cell Exosomes Improve Survival of Neural Stem Cells after Radiation Exposure

被引：0
|
作者：
M. G. Ratushnyak
Yu. P. Semochkina
E. V. Yastremsky
R. A. Kamyshinsky
机构：
[1] National Research Center “Kurchatov Institute”,
来源：
Bulletin of Experimental Biology and Medicine | 2022年 / 173卷
关键词：
neural stem cells; mesenchymal stem cells; exosomes; cell therapy; gamma radiation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The death of neural stem cells in the hippocampus during radiation therapy of brain tumors leads to neurogenesis impairment and the development of cognitive dysfunctions at delayed terms after irradiation. Exosomes secreted by stem cells can provide a protective effect on neural stem cells. We isolated and characterized exosomes from the medium conditioned by neural stem cells and mesenchymal stem cells from mouse adipose tissue and studied their efficiency in protecting irradiated neural stem cells. According to dynamic light scattering data, the exosome size varied from 44 to 68 nm for neural stem cells, and from 80 to 130 nm for mesenchymal stem cells. All exosomes carried markers CD9, CD63, and TSG101. The survival rate and clonogenic activity of neural stem cells irradiated in a dose of 1 Gy was found to increase after culturing in the presence of stem cell exosome preparations.
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页码：544 / 552
页数：8
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