A simple and accurate method for generating co-dominant markers: an application of conformation-sensitive gel electrophoresis to linkage analysis in the silkworm

被引：0
|
作者：
Y. Yasukochi
机构：
[1] National Institute of Sericultural and Entomological Science (NISES),
[2] Tsukuba,undefined
[3] Ibaraki 305-8634,undefined
[4] Japan e-mail: yaskoch@nises.affrc.go.jp; Fax: +81-298-386090,undefined
来源：
Molecular and General Genetics MGG | 1999年 / 261卷
关键词：
Key words Conformation-sensitive gel electrophoresis; Heteroduplex formation; Bombyx mori; Linkage analysis; Sequence-tagged site;
D O I：
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学科分类号：
摘要：
A simple and sensitive method for linkage analysis is described, which is based on conformation-sensitive gel electrophoresis (CSGE). Using urea-containing agarose gels or a commercially available polyacrylamide-derived matrix, 13 polymorphic markers were newly identified for known genes of the silkworm, Bombyx mori, which had been scored as monomorphic by PCR-RFLP analysis. This method for detecting polymorphisms is quite sensitive, and can be performed with inexpensive reagents and apparatus that is available in most molecular biology laboratories.
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页码：796 / 802
页数：6
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