Detection of hepatitis E virus in acute hepatitis in Siberia

被引：0

作者：

E. V. Chausov

V. A. Ternovoi

G. V. Kochneva

V. A. Svyatchenko

G. F. Sivolobova

A. A. Grazhdantseva

N. N. Kiselev

E. G. Sakharova

S. V. Netesov

机构：

[1] Vector Science Center for Virology and Biotechnology,Scientific Research Institute of Molecular Biology

[2] Municipal Clinical Infectious Disease Hospital no. 1,undefined

来源：

Molecular Genetics, Microbiology and Virology | 2007年 / 22卷 / 3期

关键词：

Acute Hepatitis; Infectious Disease Hospital; AF124407 AF459438 AF076239; AF124407 AF459438 AF076239 AF051351; Clinical Infectious Disease Hospital;

D O I：

10.3103/S089141680703007X

中图分类号：

学科分类号：

摘要：

Samples of blood serum obtained from patients with symptoms of acute hepatitis, admitted to infectious disease hospitals of Novosibirsk, Barnaul, and Irkutsk, were investigated. The serologic markers of hepatitis E virus (HEV): IgM and IgG antibodies, were determined by enzyme immunoassay (ELISA). Seropositive samples were investigated by the method of reverse transcription and the polymerase chain reaction (RTPCR). Two variants of HEV were detected, and the first human cases of HEV infection in this region were demonstrated. One of the variants was identified as genotype I, the other as genotype III of HEV. The HEV variants found were cultured in a continuous culture of green monkey kidney cells (4647). The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.

引用

页码：131 / 136

页数：5

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