The efficiency of zinc sulfate immersion bath on improved wound healing via promoting antioxidant activity, gene expression biomarkers, and skin re-epithelization in a common carp-induced wound model

被引：2

作者：

El-Adl, Mohamed ^{[1
]}

Rezk, Shaymaa ^{[2
]}

Ali, Mayar ^{[3
]}

Lashen, Samah ^{[2
]}

Fouda, Moustafa ^{[4
]}

El Sebaei, Mahmoud G. ^{[1
,5
]}

Shukry, Mustafa ^{[6
]}

Abdelkhalek, Nevien ^{[4
]}

Naiel, Mohammed A. E. ^{[7
]}

机构：

[1] Mansoura Univ, Fac Vet Med, Dept Biochem & Chem Nutr, Mansoura, Egypt

[2] Mansoura Univ, Fac Vet Med, Dept Cytol & Histol, Mansoura, Egypt

[3] Mansoura Univ, Fac Vet Med, Dept Anim Husb & Prod, Mansoura, Egypt

[4] Mansoura Univ, Fac Vet Med, Dept Internal Med Infect & Fish Dis, Mansoura, Egypt

[5] King Faisal Univ, Coll Vet Med, Dept Biomed Sci, Al Hasa, Saudi Arabia

[6] Kafrelsheikh Univ, Fac Vet Med, Dept Physiol, Kafrelsheikh 33516, Egypt

[7] Zagazig Univ, Fac Agr, Dept Anim Prod, Zagazig 44519, Egypt

来源：

APPLIED WATER SCIENCE | 2024年 / 14卷 / 02期

关键词：

Zinc sulfate; Wound healing; Oxidative stress; Skin reepithelization; Common carp; GROWTH-FACTOR-BETA; SALMO-SALAR; ATLANTIC SALMON; FISH; REPAIR; INFLAMMATION; STIMULATION; MODULATION; MOLECULES; MECHANISM;

D O I：

10.1007/s13201-023-02077-z

中图分类号：

TV21 [水资源调查与水利规划];

学科分类号：

081501 ;

摘要：

The experiment was designed to examine the influence of employing three doses of ZnSO4 on the wound healing process in partially scaled common carp. A total of 240 healthy common carp fish (52.3 +/- 0.9 g) were randomly allocated into four equal groups in triplicate (20 each). The first group left without any zinc sulfate treatment and served as a control group, while the second group through the fourth group were immersed in a zinc sulfate bath at a dose of 2.09, 1.05, and 0.53 mg/L corresponding to 1/5, 1/10, and 1/20 of 96 h LC50 of Zn, (Zn/5, Zn/10, and Zn/20, respectively). After wound induction, tissue specimens were collected within three different intervals (6 h, 24 h, 72 h, and 14 days). The results indicated that the Zn/5 fish group induced doubled folding increments in the expression of transforming growth factor (TGF)-beta 1 after 6 h compared to other groups, whereas collagen type I alpha 1 (COL1 alpha 1) and metallothionein (Met) genes exhibited a triple folding increment compared to Zn/10 and a fivefold increase compared to control after two days of wound induction. Moreover, vascular endothelial growth factor (VEGF)-A and fibroblast growth factor (FGF)-7 genes showed a dose-dependent manner of expression at all examined points after wound induction. Also, all estimated antioxidant biomarker (superoxide-dismutase, SOD; catalase, CAT; glutathione, GSH; and malonaldehyde, MDA) activities were boosted in the Zn/5 group till three days of wound induction compared to all groups. In addition, the reepithelization score and histological alteration results revealed clear improvement in the Zn/5 group, as most muscle fibers appeared regular, straight, and parallel arranged. In contrast, other groups exhibited a detectable limited area of disrupted muscle fibers. Finally, it could be concluded that the ZnSO4 immersion bath at 1/5 of the calculated LC50 effectively enhanced the healing process and skin reepithelization.

引用

页数：13