Detection of Azadinium poporum in New Zealand: the use of molecular tools to assist with species isolations

被引:0
|
作者
Kirsty F. Smith
Lesley Rhodes
D. Tim Harwood
Janet Adamson
Catherine Moisan
Rex Munday
Urban Tillmann
机构
[1] Cawthron Institute,
[2] AgResearch,undefined
[3] Ruakura Agricultural Research Centre,undefined
[4] Alfred Wegener Institute for Polar and Marine Research,undefined
来源
Journal of Applied Phycology | 2016年 / 28卷
关键词
Azaspiracids; New Zealand; Real-time PCR;
D O I
暂无
中图分类号
学科分类号
摘要
A real-time PCR assay for the detection of species from the genera Azadinium and Amphidoma (family Amphidomataceae) was developed in order to screen field samples and to aid in the isolation of azaspiracid (AZA)-producing dinoflagellates. The assay was highly specific and sensitive and allowed the rapid detection of target species. Samples collected as part of the New Zealand Marine Phytoplankton Monitoring Programme were analysed using the Amphidomataceae real-time PCR assay. Azadinium poporum was detected in New Zealand for the first time, and a culture was successfully established. Extracts of this isolate proved to be of low toxicity to mice and did not contain AZA-1, -2 or -3. Field samples will continue to be screened with the aim of identifying AZA-producing species. The Amphidomataceae real-time PCR assay will be a useful tool for monitoring programmes and taxonomic surveys worldwide.
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页码:1125 / 1132
页数:7
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