Detection of Azadinium poporum in New Zealand: the use of molecular tools to assist with species isolations
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作者:
Kirsty F. Smith
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机构:Cawthron Institute,
Kirsty F. Smith
Lesley Rhodes
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h-index: 0
机构:Cawthron Institute,
Lesley Rhodes
D. Tim Harwood
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h-index: 0
机构:Cawthron Institute,
D. Tim Harwood
Janet Adamson
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机构:Cawthron Institute,
Janet Adamson
Catherine Moisan
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h-index: 0
机构:Cawthron Institute,
Catherine Moisan
Rex Munday
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机构:Cawthron Institute,
Rex Munday
Urban Tillmann
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机构:Cawthron Institute,
Urban Tillmann
机构:
[1] Cawthron Institute,
[2] AgResearch,undefined
[3] Ruakura Agricultural Research Centre,undefined
[4] Alfred Wegener Institute for Polar and Marine Research,undefined
来源:
Journal of Applied Phycology
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2016年
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28卷
关键词:
Azaspiracids;
New Zealand;
Real-time PCR;
D O I:
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摘要:
A real-time PCR assay for the detection of species from the genera Azadinium and Amphidoma (family Amphidomataceae) was developed in order to screen field samples and to aid in the isolation of azaspiracid (AZA)-producing dinoflagellates. The assay was highly specific and sensitive and allowed the rapid detection of target species. Samples collected as part of the New Zealand Marine Phytoplankton Monitoring Programme were analysed using the Amphidomataceae real-time PCR assay. Azadinium poporum was detected in New Zealand for the first time, and a culture was successfully established. Extracts of this isolate proved to be of low toxicity to mice and did not contain AZA-1, -2 or -3. Field samples will continue to be screened with the aim of identifying AZA-producing species. The Amphidomataceae real-time PCR assay will be a useful tool for monitoring programmes and taxonomic surveys worldwide.
机构:
Cawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New ZealandCawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New Zealand
Smith, Kirsty F.
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Rhodes, Lesley
Harwood, D. Tim
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机构:
Cawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New ZealandCawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New Zealand
Harwood, D. Tim
Adamson, Janet
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机构:
Cawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New ZealandCawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New Zealand
Adamson, Janet
Moisan, Catherine
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机构:
Cawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New ZealandCawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New Zealand
Moisan, Catherine
Munday, Rex
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机构:
Ruakura Agr Res Ctr, AgRes, 10 Bisley Rd,Private Bag 3240, Hamilton, New ZealandCawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New Zealand
Munday, Rex
Tillmann, Urban
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机构:
Alfred Wegener Inst Polar & Marine Res, Am Handelshafen 12, D-27570 Bremerhaven, GermanyCawthron Inst, 98 Halifax St East,Private Bag 2, Nelson 7042, New Zealand
机构:
Hanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South KoreaHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Kim, Joo-Hwan
Tillmann, Urban
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机构:
Helmholtz Ctr Polar & Marine Res, Alfred Wegener Inst, Handelshafen 12, D-27570 Bremerhaven, GermanyHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Tillmann, Urban
Adams, Nicolaus G.
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NOAA, Northwest Fisheries Sci Ctr, Natl Marine Fisheries Serv, 2725 Montlake Blvd E, Seattle, WA 98112 USAHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Adams, Nicolaus G.
Krock, Bernd
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机构:
Helmholtz Ctr Polar & Marine Res, Alfred Wegener Inst, Handelshafen 12, D-27570 Bremerhaven, GermanyHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Krock, Bernd
Stutts, Whitney L.
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US FDA, Ctr Food Safety & Appl Nutr, 5001 Campus Dr, College Pk, MD 20740 USAHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Stutts, Whitney L.
Deeds, Jonathan R.
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US FDA, Ctr Food Safety & Appl Nutr, 5001 Campus Dr, College Pk, MD 20740 USAHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Deeds, Jonathan R.
Han, Myung-Soo
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Hanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South KoreaHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Han, Myung-Soo
Trainer, Vera L.
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NOAA, Northwest Fisheries Sci Ctr, Natl Marine Fisheries Serv, 2725 Montlake Blvd E, Seattle, WA 98112 USAHanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
机构:
Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USAWashington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USA
Schroeder, Kurtis L.
Martin, Frank N.
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USDA ARS, Salinas, CA USAWashington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USA
Martin, Frank N.
de Cock, Arthur W. A. M.
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CBS KNAW Fungal Biodivers Ctr, Utrecht, NetherlandsWashington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USA
de Cock, Arthur W. A. M.
Levesque, C. Andre
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Agr & Agri Food Canada, Cent Expt Farm, Biodivers Grp, Ottawa, ON, CanadaWashington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USA
Levesque, C. Andre
Spies, Christoffel F. J.
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机构:Washington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USA
Spies, Christoffel F. J.
Okubara, Patricia A.
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机构:
Washington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USAWashington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USA
Okubara, Patricia A.
Paulitz, Timothy C.
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机构:Washington State Univ, USDA ARS, Dis & Biol Control Res Unit, Pullman, WA 99164 USA
机构:
Univ Auckland, Dept Mol Med & Pathol, Auckland, New Zealand
Univ Auckland, Dept Surg, Auckland, New ZealandUniv Auckland, Dept Mol Med & Pathol, Auckland, New Zealand
Wright, Deborah M.
McNeill, Rob
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Univ Auckland, Sch Populat Hlth, Auckland, New ZealandUniv Auckland, Dept Mol Med & Pathol, Auckland, New Zealand
McNeill, Rob
Merrie, Arend E. H.
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机构:
Univ Auckland, Dept Surg, Auckland, New Zealand
Auckland City Hosp, Dept Surg, Colorectal Unit, Auckland, New ZealandUniv Auckland, Dept Mol Med & Pathol, Auckland, New Zealand
Merrie, Arend E. H.
Print, Cristin G.
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机构:
Univ Auckland, Dept Mol Med & Pathol, Auckland, New Zealand
Univ Auckland, Bioinformat Inst, Auckland, New ZealandUniv Auckland, Dept Mol Med & Pathol, Auckland, New Zealand