The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors

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作者
Jeroen Roose
Miranda Molenaar
Josi Peterson
Jolanda Hurenkamp
Helen Brantjes
Petra Moerer
Marc van de Wetering
Olivier Destrée
Hans Clevers
机构
[1] University Hospital,Department of Immunology
[2] Hubrecht Laboratory,undefined
[3] Netherlands Institute for Developmental Biology,undefined
来源
Nature | 1998年 / 395卷
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摘要
Tcf/Lef transcription factors mediate signalling from Wingless/Wnt proteins by recruiting Armadillo/β-catenin as a transcriptional co-activator1,2,3,4,5,6,7. However, studies of Drosophila, Xenopus and Caenorhabditis elegans have indicated that Tcf factors may also be transcriptional repressors6,8,9,10,11,12,13. Here we show that Tcf factors physically interact with members of the Groucho family of transcriptional repressors. In transient transfection assays, the Xenopus Groucho homologue XGrg-4 inhibited activation of transcription of synthetic Tcf reporter genes. In contrast, the naturally truncated Groucho-family member XGrg-5 enhanced transcriptional activation. Injection of XGrg-4 into Xenopus embryos repressed transcription of Siamois and Xnr-3, endogenous targets of β-catenin–Tcf. Dorsal injection of XGrg-4 had a ventralizing effect on Xenopus embryos. Secondary-axis formation induced by a dominant-positive Armadillo–Tcf fusion protein was inhibited by XGrg-4 and enhanced by XGrg-5. These data indicate that expression of Tcf target genes is regulated by a balance between Armadillo and Groucho.
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页码:608 / 612
页数:4
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