An efficient and quick protocol for in vitro multiplication of snake plant, Sansevieria trifasciata var. Laurentii [Prain]

An efficient protocol was developed for quick propagation of snake plant under in vitro conditions. Leaf segments sized 1 cm were surface sterilized and inoculated on Murashige and Skoog media supplemented with 3% sucrose, 0.8% agar, and various concentrations of indole-3-butyric acid (1 to 10 mg/L). Cultures were maintained for 4 to 5 weeks at standard conditions to allow root induction and elongation. Shoot induction was triggered upon altering the daytime culture room temperature to 37 ± 2 °C. Multiple shoots were produced at higher IBA concentrations. Another 5 weeks later, individual plantlets were excised and hardened for 2 weeks in soil preparation contained in small cups before transferring to 30 × 20 × 20 cm sized pots. We discuss the probable events effectuating unusual shoot regeneration at relatively higher temperatures in media without supplementing any cytokinins.