SERS-based plasmonic nanobiosensing in single living cells

被引：0
|
作者：
Jonathan P. Scaffidi
Molly K. Gregas
Victoria Seewaldt
Tuan Vo-Dinh
机构：
[1] Duke University,Department of Biomedical Engineering
[2] Duke University,Fitzpatrick Institute for Photonics
[3] Duke School of Medicine,Division of Medical Oncology
[4] Duke University,Department of Chemistry
来源：
Analytical and Bioanalytical Chemistry | 2009年 / 393卷
关键词：
Fiber-optic nanoprobe; Intracellular pH measurement;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized “normal” human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level.
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页码：1135 / 1141
页数：6
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