Modulation of human interferon-γ biosynthesis by antisense oligodeoxynucleotides

被引：0
|
作者：
Christine M. A. Boeve
Marc DE Ley
机构：
[1] Katholieke Universiteit Leuven,Laboratory for Biochemistry
来源：
Molecular Biotechnology | 2000年 / 14卷
关键词：
Antisense oligodeoxynucleotides; inhibition of gene expression; interferon-γ; lymphocyte;
D O I：
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学科分类号：
摘要：
We investigated the inhibition of human interferon-γ (HuIFN-γ) production in cultures of lymphocytes with the use of the antisense strategy. Out of a series of antisense oligodeoxynucleotides (ODN) complementary to different regions of the HuIFN-γ gene, a 16-mer specific for a sequence including the translation initiation codon was the most effective. Here we describe a detailed protocol for the isolation of lymphocytes from buffy coats, the rational design of antisense ODN, and the monitoring of HuIFN-γ production of the antisense ODN-treated cells.
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页码：157 / 163
页数：6
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