Biogenic Preparation and Characterization of ZnO Nanoparticles from Natural Polysaccharide Azadirachta indica .L. (neem gum) and its Clinical Implications

In the present study, phytochemical analysis, synthesis and characterization of zinc oxide nanoparticles (NG-ZnO NPs) from neem gum as a capping agent. GC–MS analysis confirms the phytochemical oxadiazol-2yl-benzothiazole-(21.5%) on the synthesized ZnO nanoparticles. Various sophisticated analytical instrumentations characterized the morphological and crystalline behaviour of NG-ZnO NPs. The analytical results confirm the spherical crystals of NG-ZnO NPs in size range of 60–80 nm size. The NG-ZnO NPs shown significantly efficient antibacterial and antibiofilm activity against P. aeruginosa at 100 µg/mL. Further, in vitro study on human liver cancer cell line (Hep G2) indicated significantly inhibited the cell proliferation at 100 µg/mL in 24 h. Also, NG-ZnO NPs have also shown potent mosquito larvicidal activity on C. quinquefasciatus at 100 µg/mL. Based on findings, it is recommended that neem gum capped ZnO NPs is potential nano biomedical agents to be used in new drug formulations.