Partial extraction and identification of phenolics in Amla (Emblica officinalis) seed coat powder

RSM was used to determine the optimum conditions for maximum recovery of phenolics from amla seed coat powder. At optimum conditions, TPC (total phenolic content) content and % DPPH free radical scavenging activity of extract were found to be 7.02 mg GAE / mL of extract and 42.69 %, respectively. Phenolics were extracted in water, diethyl ether, butanol and ethyl acetate. % DPPH free radical scavenging (61.07 %) activity was maximum in ethyl acetate fraction. The major phenolics in ethyl acetate extract were gallic, catechin, caffeic, quercetin, p-coumaric, vanillic and chlorogenic acids. Ethyl acetate extract was further fractionated with six fractions using methanol: water by column chromatography. Fraction V showed highest DPPH scavenging activity. The phenolics in different fractions of ethyl acetate extract were also estimated by GC-MS after derivatization. Caeffic, p-coumaric, hydroxy caeffic, vanillic, gallic and p-hydroxybenzoic acid were identified. Other components like tetracontane-1,40-diol and octadecanoic acid could also be identified.