Separation of apoptotic cells using a microfluidic device

被引：0
|
作者：
Hyun-Seok Kim
Oh-Taek Son
Kun-Hong Kim
Sang-Hyeob Kim
Sunglyul Maeng
Hyo-Il Jung
机构：
[1] Yonsei University,School of Mechanical Engineering
[2] Yonsei Medical School,undefined
[3] Cambridge-ETRI Joint R&D Centre,undefined
来源：
Biotechnology Letters | 2007年 / 29卷
关键词：
Apoptotic cell; Microfluidic device; Permanent magnet;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A highly sensitive microfluidic device has been developed to separate apoptotic cells. Apoptotic Jurkat cells were selectively labeled with magnetic beads (0.8 μm diam) using the C2A protein which recognizes phosphatidylserine. The cell mixture was flowed through a microfluidic channel and apoptotic cells were separated by a 0.3 T permanent magnet. Separations using our device showed 96% agreement with those of a commercial flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.
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页码：1659 / 1663
页数：4
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