Preparation and characterization of monoclonal antibodies to the cholera toxin

被引：0
|
作者：
E. E. Petrova
R. L. Komaleva
O. E. Lakhtina
L. V. Samokhvalova
N. A. Kalinina
N. S. Shoshina
A. Yu. Rubina
M. A. Filippova
Yu. V. Vertiev
T. I. Valyakina
Ye. V. Grishin
机构：
[1] Russian Academy of Sciences,Shemyakin
[2] Russian Academy of Sciences,Ovchinnikov Institute of Bioorganic Chemistry
[3] Russian Academy of Medical Sciences,Engelhardt Institute of Molecular Biology
来源：
Russian Journal of Bioorganic Chemistry | 2009年 / 35卷
关键词：
enzyme immunoassay; sandwich assay; biological microchip; B subunit of cholera toxin; cholera toxin; monoclonal antibodies;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA.
引用
收藏
页码：326 / 334
页数：8
相关论文
共 50 条
  • [1] Preparation and characterization of monoclonal antibodies to the cholera toxin
    Petrova, E. E.
    Komaleva, R. L.
    Lakhtina, O. E.
    Samokhvalova, L. V.
    Kalinina, N. A.
    Shoshina, N. S.
    Rubina, A. Yu.
    Filippova, M. A.
    Vertiev, Yu. V.
    Valyakina, T. I.
    Grishin, Ye. V.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2009, 35 (03) : 326 - 334
  • [2] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO CHOLERA-TOXIN
    REMMERS, EF
    COLWELL, RR
    GOLDSBY, RA
    INFECTION AND IMMUNITY, 1982, 37 (01) : 70 - 76
  • [3] CHARACTERIZATION OF SEVERAL MONOCLONAL-ANTIBODIES AGAINST CHOLERA-TOXIN
    IMAGAWA, Y
    MAEDA, T
    TOKUNAGA, E
    KUDOU, K
    MURAOKA, T
    OHTOMO, N
    JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1984, 37 (04): : 204 - 204
  • [4] Characterization and utility of two monoclonal antibodies to cholera toxin B subunit
    Noel Verjan Garcia
    Ian Carlosalberto Santisteban Celis
    Matthew Dent
    Nobuyuki Matoba
    Scientific Reports, 13
  • [5] Characterization and utility of two monoclonal antibodies to cholera toxin B subunit
    Verjan Garcia, Noel
    Santisteban Celis, Ian Carlosalberto
    Dent, Matthew
    Matoba, Nobuyuki
    SCIENTIFIC REPORTS, 2023, 13 (01)
  • [6] Production and purification of monoclonal and polyclonal antibodies against cholera toxin
    Chou, SF
    HYBRIDOMA AND HYBRIDOMICS, 2004, 23 (04): : 258 - 261
  • [7] MONOCLONAL-ANTIBODIES TO THE A-SUBUNIT OF CHOLERA-TOXIN
    KUROSKY, A
    DUFFY, LK
    DENNEY, RM
    FEDERATION PROCEEDINGS, 1982, 41 (04) : 1390 - 1390
  • [8] Preparation and characterization of monoclonal antibodies to thrombomodulin
    Tsuneyoshi, N
    Fukudome, K
    Higuchi, K
    Kimoto, M
    HYBRIDOMA, 2005, 24 (04): : 189 - 196
  • [9] CHOLERA-TOXIN A SUBUNIT - MONOCLONAL-ANTIBODIES AND BIOLOGICAL-ACTIVITY
    PEARSON, SD
    MERRYMAN, LS
    DUFFY, LK
    KUROSKY, A
    JOURNAL OF TOXICOLOGY-TOXIN REVIEWS, 1986, 5 (02): : 192 - 192
  • [10] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO TETANUS TOXIN
    SHEPPARD, AJ
    CUSSELL, D
    HUGHES, M
    INFECTION AND IMMUNITY, 1984, 43 (02) : 710 - 714
12345