Protection of normal human reconstructed epidermis from UV by catalase overexpression

被引：0
|
作者：
H R Rezvani
M Cario-André
C Pain
C Ged
H deVerneuil
A Taïeb
机构：
[1] Inserm E 0217,Department of Dermatology
[2] University Victor Segalen Bordeaux 2,undefined
[3] CHU Bordeaux,undefined
[4] Hôpital St André,undefined
来源：
Cancer Gene Therapy | 2007年 / 14卷
关键词：
gene transfer; superoxide dismutase; photoprotection; lentiviral vector;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Reactive oxygen species (ROS) generated by ultraviolet (UV) irradiation are counterbalanced by endogenous antioxidant systems. To test the hypothesis of a novel photoprotective approach, we irradiated epidermis reconstructed with normal human keratinocytes overexpressing sustainably lentivirus-mediated catalase (CAT), copper/zinc superoxide dismutase (CuZnSOD) or manganese superoxide dismutase (MnSOD) enzymes. We found that following UVB irradiation there was a marked decrease in sunburn cell formation, caspase-3 activation and p53 accumulation in human reconstructed epidermis overexpressing CAT. Moreover, UVA-induced hypertrophy and DNA oxidation (8-oxodeoxyguanosine) were decreased by CAT overexpression. These effects were not achieved by overexpression of CuZnSOD or MnSOD. In conclusion, vector-mediated CAT overexpression could be a promising photoprotective tool against deleterious effects of UV irradiation such skin cancer especially in monogenic/polygenic photosensitive disorders characterized by ROS accumulation.
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页码：174 / 186
页数：12
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