Determination of Catechin and Epicatechin in an Extract from Uncaria tomentosa Bark by TLC with Chemically Modified Stationary Phases

被引:0
|
作者
Izabela Fecka
Wojciech Cisowski
Maria Luczkiewicz
机构
[1] Wroclaw Medical University,Department of Pharmacognosy
[2] Gdansk Medical University,Department of Pharmacognosy
关键词
Catechin; Epicatechin; SPE–HPTLC;
D O I
暂无
中图分类号
学科分类号
摘要
The conditions used for TLC and HPTLC analysis of catechins have been optimized. Chemically modified stationary phases (HPTLC CN, HPTLC NH2, and HPTLC RP-18W) were used for qualitative examination of polyphenol fractions obtained by an SPE–RP 18 method from Uncaria tomentosa bark. Although the best separation of catechin from epicatechin was achieved on octadecyl plates, silica gel plates are good enough for preliminary investigations of crude plant extracts.
引用
收藏
页码:405 / 408
页数:3
相关论文
共 9 条
  • [1] Determination of catechin and epicatechin in an extract from Uncaria tomentosa bark by TLC with chemically modified stationary phases
    Fecka, I
    Cisowski, W
    Luczkiewicz, M
    JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2001, 14 (06) : 405 - 408
  • [2] Determination of catechin and epicatechin in an extract from Uncaria tomentosa bark by TLC with chemically modified stationary phases
    Fecka, Izabela
    Cisowski, Wojciech
    Luczkiewicz, Maria
    Journal of Planar Chromatography - Modern TLC, 2001, 14 (06): : 405 - 408
  • [3] TLC Separation of Uncaria tomentosa Alkaloids on Chemically Modified Stationary Phases
    Michał Glensk
    Beata Żbikowska
    Wojciech Cisowski
    JPC – Journal of Planar Chromatography – Modern TLC, 2004, 17 : 14 - 17
  • [4] TLC separation of Uncaria tomentosa alkaloids on chemically modified stationary phases
    Glensk, M
    Zbikowska, B
    Cisowski, W
    JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2004, 17 (01) : 14 - 17
  • [5] Influence of water extract from Uncaria tomentosa bark on ultrastructure of Capsicum
    Tykarska, Teresa
    Zobel, Alicja
    Nowakowska, Julita
    Gulewicz, Krzysztof
    Kuvas, Mieczyslaw
    ALLELOPATHY: NEW CONCEPTS AND METHODOLOGY, 2007, : 317 - +
  • [6] TLC determination of tannins and flavonoids in extracts from some Erodium species using chemically modified stationary phases
    Fecka, I
    Cisowski, W
    JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2002, 15 (06) : 429 - 432
  • [7] TLC Determination of Tannins and Flavonoids in Extracts from Some Erodium Species using Chemically Modified Stationary Phases
    Izabela Fecka
    Wojciech Cisowski
    JPC – Journal of Planar Chromatography – Modern TLC, 2002, 15 (6): : 429 - 432
  • [8] Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques
    Aguinaga, Jefferson Yunis
    Claudiano, Gustavo S.
    Marcusso, Paulo F.
    Ikefuti, Cynthia
    Ortega, George G.
    Eto, Silas F.
    da Cruz, Claudinei
    Moraes, Juliet R. E.
    Moraes, Flavio R.
    Fernandes, Joao B. K.
    JOURNAL OF TOXICOLOGY, 2014, 2014
  • [9] Changes in chromosome structure, mitotic activity and nuclear DNA content from cells of Allium Test induced by bark water extract of Uncaria tomentosa (Willd.) DC
    Kuras, Mieczyslaw
    Nowakowska, Julita
    Sliwinska, Elwira
    Pilarski, Radoslaw
    Ilasz, Renata
    Tykarska, Teresa
    Zobel, Alicja
    Gulewicz, Krzysztof
    JOURNAL OF ETHNOPHARMACOLOGY, 2006, 107 (02) : 211 - 221