Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

被引：0

作者：

Martin Kváč

Bohumil Sak

Dagmar Hanzlíková

Jiřina Kotilová

Dana Květoňová

机构：

[1] Biology Centre of the Academy of Sciences of the Czech Republic,Institute of Parasitology

[2] University of South Bohemia in České Budějovice,Faculty of Agriculture

来源：

Parasitology Research | 2009年 / 104卷

关键词：

Cryptosporidiosis; Cryptosporidium Oocyst; Cryptosporidium Infection; Polymerase Chain Reaction Restriction Fragment; Reaction Restriction Fragment Length Polymorphism;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal RNA (SSU rRNA) and GP60 partial genes and polymerase chain reaction restriction fragment length polymorphism of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two sows (10%). Twenty-one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and Cryptosporidium suis in finishers were found. Both sows were infected with the Cryptosporidium parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.

引用

页码：425 / 428

页数：3

共 50 条

[1] Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic
Kvac, Martin
Sak, Bohumil
Hanzlikova, Dagmar
Kotilova, Jirina
Kvetonova, Dana
[J]. PARASITOLOGY RESEARCH, 2009, 104 (02) : 425 - 428
[2] Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic
Ondrackova, Zuzana
Kvac, Martin
Sak, Bohumil
Kvetonova, Dana
Rost, Michael
[J]. VETERINARY PARASITOLOGY, 2009, 165 (1-2) : 141 - 144
[3] Molecular characterization of Cryptosporidium isolates from pigs in Zaragoza (northeastern Spain)
Suarez-Luengas, Laura
Clavel, Antonio
Quilez, Joaquin
Pilar Goni-Cepero, Maria
Torres, Eucaris
Sanchez-Acedo, Caridad
del Cacho, Emilio
[J]. VETERINARY PARASITOLOGY, 2007, 148 (3-4) : 231 - 235
[4] Rock-inhabiting cyanoprokaryota from South Bohemia (Czech Republic)
Hauer, Tomas
[J]. NOVA HEDWIGIA, 2007, 85 (3-4) : 379 - 392
[5] Age-related and housing-dependence of Cryptosporidium infection of calves from dairy and beef herds in South Bohemia, Czech Republic
Kvác, M
Kouba, M
Vítovec, J
[J]. VETERINARY PARASITOLOGY, 2006, 137 (3-4) : 202 - 209
[6] A multicarpellate fruit from Late Cretaceous sediments of South Bohemia, Czech Republic
Hermanova, Zuzana
Cepickova, Jana
Kvacek, Jiri
von Balthazar, Maria
Schonenberger, Jurg
[J]. PALAEONTOLOGIA ELECTRONICA, 2022, 25 (01)
[7] Detection and partial molecular characterization of PPV recombinant isolates in locality "Lipov" (South Moravia, Czech Republic)
Gadiou, S.
Safarova, D.
Navratil, M.
[J]. PROCEEDINGS OF THE TWENTIETH INTERNATIONAL SYMPOSIUM ON VIRUS AND VIRUS-LIKE DISEASES OF TEMPERATE FRUIT CROPS - FRUIT TREE DISEASES, 2008, (781): : 243 - 247
[8] Molecular characterization of Cryptosporidium isolates from calves in Argentina
Tomazic, Mariela L.
Maidana, Jimena
Dominguez, Mariana
Louge Uriarte, Enrique
Galarza, Roxana
Garro, Carlos
Florin-Christensen, Monica
Schnittger, Leonhard
[J]. VETERINARY PARASITOLOGY, 2013, 198 (3-4) : 382 - 386
[9] Molecular characterization of Cryptosporidium isolates from humans in Ethiopia
Adamu, Haileeyesus
Petros, Beyene
Hailu, Asrat
Petry, Franz
[J]. ACTA TROPICA, 2010, 115 (1-2) : 77 - 83
[10] Molecular characterization of Cryptosporidium isolates from humans in Ethiopia
Adamu, H.
Petros, B.
Hailu, A.
Petry, F.
[J]. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2009, 299 : 11 - 11

← 1 2 3 4 5 →