Small molecules enable highly efficient neuronal conversion of human fibroblasts

被引：0

作者：

Ladewig J. ^{[1
]}

Mertens J. ^{[1
]}

Kesavan J. ^{[1
]}

Doerr J. ^{[1
]}

Poppe D. ^{[1
]}

Glaue F. ^{[1
]}

Herms S. ^{[2
]}

Wernet P. ^{[3
]}

Kögler G. ^{[3
]}

Müller F.-J. ^{[4
]}

Koch P. ^{[1
]}

Brüstle O. ^{[1
]}

机构：

[1] Institute of Reconstructive Neurobiology, Life and Brain Center, University of Bonn, Bonn

[2] Institute of Human Genetics, Life And Brain Center, University of Bonn, Bonn

[3] Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Medical Centre, Düsseldorf

[4] Department of Psychiatry and Psychotherapy, Centre for Integrative Psychiatry ZIP-Kiel, University Hospital Schleswig Holstein, Kiel

来源：

Nature Methods | 2012年 / 9卷 / 6期

关键词：

D O I：

10.1038/nmeth.1972

中图分类号：

学科分类号：

摘要：

Forced expression of proneural transcription factors has been shown to direct neuronal conversion of fibroblasts. Because neurons are postmitotic, conversion efficiencies are an important parameter for this process. We present a minimalist approach combining two-factor neuronal programming with small molecule-based inhibition of glycogen synthase kinase-3β and SMAD signaling, which converts postnatal human fibroblasts into functional neuron-like cells with yields up to >200% and neuronal purities up to >80%. © 2012 Nature America, Inc. All rights reserved.

引用

页码：575 / 578

页数：3

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