Impedimetric genosensors for the detection of DNA hybridization

被引：0
|
作者：
A. Bonanni
M. J. Esplandiu
M. I. Pividori
S. Alegret
M. del Valle
机构：
[1] Autonomous University of Barcelona,Sensors and Biosensors Group, Department of Chemistry
来源：
Analytical and Bioanalytical Chemistry | 2006年 / 385卷
关键词：
DNA biosensor; Impedance; Capacitance; Unlabelled DNA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay.
引用
收藏
页码：1195 / 1201
页数：6
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