High throughput isolation of microsatellites in hop (Humulus lupulus L.)

被引：0
|
作者：
Jernej Jakše
Branka Javornik
机构：
[1] University of Ljubljana,Centre for Plant Biotechnology and Breeding, Biotechnical Faculty
来源：
Plant Molecular Biology Reporter | 2001年 / 19卷
关键词：
enriched libraries; hop; microsatellites; molecular markers;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Procedures to generate usable microsatellite marker sequences should be optimized for cost-effectiveness in each species. For hop, we have used a cocktail of several restriction enzymes to digest the genomic DNA. This is followed by capture of microsatellite-containing sequences with long microsatellite probes attached to a membrane. The enrichment level for GA and GT libraries was 37% and 35%, respectively, and 100% of the clones contained microsatellite sequences. Libraries can be generated from genomic DNA in approximately 10 d.
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页码：217 / 226
页数：9
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