Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies

被引：0

作者：

Zhang X. ^{[1
]}

Guo C. ^{[2
]}

Chen Y. ^{[1
]}

Shulha H.P. ^{[3
]}

Schnetz M.P. ^{[1
]}

LaFramboise T. ^{[1
]}

Bartels C.F. ^{[1
]}

Markowitz S. ^{[4
]}

Weng Z. ^{[3
,5
]}

Scacheri P.C. ^{[1
]}

Wang Z. ^{[1
,6
]}

机构：

[1] Department of Genetics, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106

[2] Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, OH 44106

[3] Biomedical Engineering Department, Boston University, Boston, MA 02215

[4] Department of Medicine, Case Western Reserve University, University Hospitals of Cleveland, Cleveland

[5] Bioinformatics Program, Boston University, Boston, MA 02215

[6] Genomic Medicine Institute, Cleveland Clinic Foundation, Cleveland, OH 44195

来源：

Nature Methods | 2008年 / 5卷 / 2期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth1170

中图分类号：

学科分类号：

摘要：

We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.

引用

页码：163 / 165

页数：2

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