Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

被引：0

作者：

Ting Lei

Sandrine Jacob

Imen Ajil-Zaraa

Jean-Bernard Dubuisson

Olivier Irion

Marisa Jaconi

Anis Feki

机构：

[1] Laboratory of Stem Cell Research,Department of Obstetrics and Gynecology

[2] Geneva University Hospitals,Department of Obstetrics and Gynecology

[3] Geneva University Hospitals,Department of Pathology and Immunology

[4] Faculty of Medicine,undefined

[5] Geneva University,undefined

来源：

Cell Research | 2007年 / 17卷

关键词：

embryonic stem cells; inner cell mass; derivation; feeder cells; xeno-free; differentiation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications.

引用

页码：682 / 688

页数：6

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