We prove that the minimal cardinality of a semitransitive subsemigroup in the singular part \documentclass[12pt]{minimal}
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$\mathcal{I}_{n}\setminus \mathcal{S}_{n}$
\end{document} of the symmetric inverse semigroup \documentclass[12pt]{minimal}
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$\mathcal{I}_{n}$
\end{document} is 2n−p+1, where p is the greatest proper divisor of n, and classify all semitransitive subsemigroups of this minimal cardinality.