Functional synapse formation between compartmentalized cortical neurons cultured inside microfluidic devices

被引:0
|
作者
Sanjeev Kumar Mahto
Hue-sup Song
Seog Woo Rhee
机构
[1] Kongju National University,Department of Chemistry
[2] Kongju National University,Division of Advanced Materials Engineering
来源
BioChip Journal | 2011年 / 5卷
关键词
Microfluidics; Multicompartments; Cortical neurons; Synapse formation; Real-time monitoring;
D O I
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中图分类号
学科分类号
摘要
In this work, the formation of functional synapses between compartmentalized cortical neurons cultured inside three-compartment microfluidic devices in a controlled fashion is described. The proposed device can direct axons in an isolated compartment and, thus, facilitates isolated axons forming functional synapses with dendrites of other neurons in an isolated microenvironment. This microfluidic approach allows continuous real-time monitoring of neuronal processes and fluorescently tagged biomolecules involved in synapse formation, and provides an easy, simple, cost effective, and efficient method to develop and manipulate synapses in an isolated microenvironment without using surface patterning techniques or electrical stimulation. The results presented here suggest that this microfluidic approach could be used as an alternative method for the formation of functional synapses and their exhaustive examinations.
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页码:289 / 298
页数:9
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