Production and characterization of xylanases of a Bacillus strain isolated from soil

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作者
Banu Avcioglu
Banu Eyupoglu
Ufuk Bakir
机构
[1] Middle East Technical University,Biotechnology Division
[2] Middle East Technical University,Chemical Engineering Department
关键词
characterization; paper pulp; production; xylanase;
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摘要
Xylanase production was performed by growing a Bacillus isolate on agricultural by-products, wheat straw, wheat bran, corn cobs and cotton bagasse. A maximum xylanase activity of 180 U/ml was obtained together with a cellulase activity of 0.03 U/ml on 4 (w/v) corn cobs. Electrophoretic analysis showed the presence of three endo-β-1, 4-xylanases having molecular weights of about 22, 23 and 40 kDa. Xylanolytic activity was stable up to 50 °C in the pH range of 4.5–10 and the highest activity was observed at 70 °C and pH 6.5.
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页码:65 / 68
页数:3
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