Faster STORM using compressed sensing

被引：0
|
作者：
Zhu L. [1 ]
Zhang W. [2 ]
Elnatan D. [3 ]
Huang B. [2 ,3 ,4 ]
机构：
[1] Nuclear and Radiological Engineering and Medical Physics Programs, George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA
[2] Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA
[3] Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA
[4] Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA
来源：
Nature Methods | 2012年 / 9卷 / 7期
关键词：
D O I：
10.1038/nmeth.1978
中图分类号：
学科分类号：
摘要：
In super-resolution microscopy methods based on single-molecule switching, the rate of accumulating single-molecule activation events often limits the time resolution. Here we developed a sparse-signal recovery technique using compressed sensing to analyze images with highly overlapping fluorescent spots. This method allows an activated fluorophore density an order of magnitude higher than what conventional single-molecule fitting methods can handle. Using this method, we demonstrated imaging microtubule dynamics in living cells with a time resolution of 3 s. © 2012 Nature America, Inc. All rights reserved.
引用
收藏
页码：721 / 723
页数：2
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