Synthesis of CdS Nanoparticle and Reveal Its Effect on Reproductive System of Male Albino Rats

This is a controlled research laboratory study (conducted in the research laboratory of the University of Rajasthan) which shows synthesis of CdS nanoparticle (NPs) and its effects on male reproductive system. Albino Wistar male rats (Rattus norvegicus), healthy fertile 3-4 months old weighing about 150-180 g were used for experimentation. NPs synthesis by chemical method and injected intra-peritoneal in healthy fertile male rats at the dose level of 2. 5 mg rat-1 day-1 for 30 days. XRD and TEM analysis of NPs were performed for size measurement. Animals were killed 24 h after withdrawal of treatment, and sperm, haematological, serum, tissue biochemistry, and histopathological analyses were performed. NPs were synthesised and their size confirmed by XRD-TEM image. Sperm analysis altered, hormonal, biochemical, and histological changes support its anti-spermatogenic effect on reproductive organ. XRD pattern and TEM observation showed size of NPS which was estimated at 20 nm. Decrease in sperm density and motility, fertility index, testes and accessory organ weight, tissue protein, and glycogen contents in testes were observed. Increased cholesterol level and decrease testosterone level showed its adverse effect on fertility. Histological degradation in testes is clearly visible. No significant alteration in body and vital organs weight was found. NPs were synthesised successfully and revealed contraceptive activity in male rats. © 2012 Springer Science+Business Media New York.