Antifouling potential of bacteria isolated from a marine biofilm

被引:0
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作者
Min Gao
Ke Wang
Rongguo Su
Xuzhao Li
Wei Lu
机构
[1] Ocean University of China,Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education
[2] Marine Chemical Research Institute,undefined
来源
关键词
marine biofilm; bacteria; antifouling activity; fatty acids;
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摘要
Marine microorganisms are a new source of natural antifouling compounds. In this study, two bacterial strains, Kytococcus sedentarius QDG-B506 and Bacillus cereus QDG-B509, were isolated from a marine biofilm and identified. The bacteria fermentation broth could exert inhibitory effects on the growth of Skeletonema costatum and barnacle larvae. A procedure was employed to extract and identify the antifouling compounds. Firstly, a toxicity test was conducted by graduated pH and liquid-liquid extraction to determine the optimal extraction conditions. The best extraction conditions were found to be pH 2 and 100% petroleum ether. The EC50 value of the crude extract of K. sedentarius against the test microalgae was 236.7 ± 14.08 μg mL−1, and that of B. cereus was 290.6 ± 27.11 μg mL−1. Secondly, HLB SPE columns were used to purify the two crude extracts. After purification, the antifouling activities of the two extracts significantly increased: the EC50 of the K. sedentarius extract against the test microalgae was 86.4 ± 3.71 μg mL−1, and that of B. cereus was 92.6 ± 1.47 μg mL−1. These results suggest that the metabolites produced by the two bacterial strains are with high antifouling activities and they should be fatty acid compounds. Lastly, GC-MS was used for the structural elucidation of the compounds. The results show that the antifouling compounds produced by the two bacterial strains are myristic, palmitic and octadecanoic acids.
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页码:799 / 804
页数:5
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