Cyanobacterial alkaline/neutral invertases. Origin of sucrose hydrolysis in the plant cytosol?

The aim of this work was to investigate the occurrence of invertase (Inv) in cyanobacteria. We describe the first isolation and characterization of prokaryotic alkaline/neutral Inv (A/N-Inv) genes. Two genes (invA and invB) were identified in Anabaena sp. PCC 7120, which share about 50–56% identity with plant A/N-Inv and encode proteins of about 53–55 kDa. The identification of these proteins was confirmed by biochemical and immunological studies with recombinant proteins and with the enzymes isolated from Anabaena cells. Expression analysis supported the important role of A/N-Inv in nitrogen-fixing growth conditions. Nevertheless, A/N-Inv activities were shown in all filamentous and unicellular cyanobacteria investigated, regardless of their capacity to fix dinitrogen. Searches in complete sequenced genomes showed that A/N-Inv homologues are restricted to cyanobacterial species and plants. In particular, filamentous nitrogen-fixing strains display two A/N-Inv genes and unicellular strains have only one. Phylogenetic analysis leads us to suggest that modern plant A/N-Inv might have originated from an orthologous ancestral gene after the endosymbiotic origin of chloroplasts.