Study of Genetic Stability of Human Bone Marrow Multipotent Mesenchymal Stromal Cells

被引：0

作者：

V. A. Nikitina

E. Yu. Osipova

L. D. Katosova

S. A. Rumyantsev

E. V. Skorobogatova

T. V. Shamanskaya

N. P. Bochkov

机构：

[1] the Russian Academy of Medical Sciences,Medical Genetic Research Center

[2] the Russian Ministry of Health and Social Development,Federal Center of Pediatric Hematology, Oncology, and Immunology

来源：

Bulletin of Experimental Biology and Medicine | 2011年 / 150卷

关键词：

multipotent mesenchymal stromal cells; karyotype; aneuploidy; FISH;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells were studied. Despite the reduction of proliferative activity by passages 11–12, the cells retained the characteristic immunophenotype. The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during in vitro culturing.

引用

页码：627 / 631

页数：4

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