Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging

被引:0
|
作者
Etsuo A Susaki
Kazuki Tainaka
Dimitri Perrin
Hiroko Yukinaga
Akihiro Kuno
Hiroki R Ueda
机构
[1] The University of Tokyo,Department of Systems Pharmacology
[2] Japan Agency for Medical Research and Development (AMED) – Core Research for Evolutionary Science and Technology (CREST),Department of Anatomy and Embryology
[3] Laboratory for Synthetic Biology,undefined
[4] RIKEN Quantitative Biology Center (QBiC),undefined
[5] School of Electrical Engineering and Computer Science,undefined
[6] Science and Engineering Faculty,undefined
[7] Queensland University of Technology (QUT),undefined
[8] Faculty of Medicine,undefined
[9] University of Tsukuba,undefined
[10] PhD Program in Human Biology,undefined
[11] School of Integrative and Global Majors,undefined
[12] University of Tsukuba,undefined
来源
Nature Protocols | 2015年 / 10卷
关键词
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中图分类号
学科分类号
摘要
This protocol describes how to perform CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis), a simple and efficient method for organ clearing, imaging by light-sheet microscopy, and quantitative imaging analysis.
引用
收藏
页码:1709 / 1727
页数:18
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