Single-cell dissection of intratumoral heterogeneity and lineage diversity in metastatic gastric adenocarcinoma

被引:0
|
作者
Ruiping Wang
Minghao Dang
Kazuto Harada
Guangchun Han
Fang Wang
Melissa Pool Pizzi
Meina Zhao
Ghia Tatlonghari
Shaojun Zhang
Dapeng Hao
Yang Lu
Shuangtao Zhao
Brian D. Badgwell
Mariela Blum Murphy
Namita Shanbhag
Jeannelyn S. Estrella
Sinchita Roy-Chowdhuri
Ahmed Adel Fouad Abdelhakeem
Yuanxin Wang
Guang Peng
Samir Hanash
George A. Calin
Xingzhi Song
Yanshuo Chu
Jianhua Zhang
Mingyao Li
Ken Chen
Alexander J. Lazar
Andrew Futreal
Shumei Song
Jaffer A. Ajani
Linghua Wang
机构
[1] The University of Texas MD Anderson Cancer Center,Department of Genomic Medicine
[2] The University of Texas MD Anderson Cancer Center,Department of Gastrointestinal Medical Oncology
[3] The University of Texas MD Anderson Cancer Center,Department of Bioinformatics and Computational Biology
[4] The University of Texas MD Anderson Cancer Center,Department of Nuclear Medicine
[5] The University of Texas MD Anderson Cancer Center,Department of Surgical Oncology
[6] The University of Texas MD Anderson Cancer Center,Department of Pathology
[7] The University of Texas MD Anderson Cancer Center,Department of Clinical Cancer Prevention
[8] The University of Texas MD Anderson Cancer Center,Department of Experimental Therapeutics
[9] University of Pennsylvania School of Medicine,Department of Biostatistics and Epidemiology
[10] The University of Texas MD Anderson Cancer Center,Department of Translational Molecular Pathology
[11] The University of Texas MD Anderson Cancer Center,UTHealth Graduate School of Biomedical Sciences
[12] Kumamoto University,Department of Gastroenterological Surgery
来源
Nature Medicine | 2021年 / 27卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Intratumoral heterogeneity (ITH) is a fundamental property of cancer; however, the origins of ITH remain poorly understood. We performed single-cell transcriptome profiling of peritoneal carcinomatosis (PC) from 15 patients with gastric adenocarcinoma (GAC), constructed a map of 45,048 PC cells, profiled the transcriptome states of tumor cell populations, incisively explored ITH of malignant PC cells and identified significant correlates with patient survival. The links between tumor cell lineage/state compositions and ITH were illustrated at transcriptomic, genotypic, molecular and phenotypic levels. We uncovered the diversity in tumor cell lineage/state compositions in PC specimens and defined it as a key contributor to ITH. Single-cell analysis of ITH classified PC specimens into two subtypes that were prognostically independent of clinical variables, and a 12-gene prognostic signature was derived and validated in multiple large-scale GAC cohorts. The prognostic signature appears fundamental to GAC carcinogenesis and progression and could be practical for patient stratification.
引用
收藏
页码:141 / 151
页数:10
相关论文
共 50 条
  • [1] Single-cell dissection of intratumoral heterogeneity and lineage diversity in metastatic gastric adenocarcinoma
    Wang, Ruiping
    Dang, Minghao
    Harada, Kazuto
    Han, Guangchun
    Wang, Fang
    Pool Pizzi, Melissa
    Zhao, Meina
    Tatlonghari, Ghia
    Zhang, Shaojun
    Hao, Dapeng
    Lu, Yang
    Zhao, Shuangtao
    Badgwell, Brian D.
    Blum Murphy, Mariela
    Shanbhag, Namita
    Estrella, Jeannelyn S.
    Roy-Chowdhuri, Sinchita
    Abdelhakeem, Ahmed Adel Fouad
    Wang, Yuanxin
    Peng, Guang
    Hanash, Samir
    Calin, George A.
    Song, Xingzhi
    Chu, Yanshuo
    Zhang, Jianhua
    Li, Mingyao
    Chen, Ken
    Lazar, Alexander J.
    Futreal, Andrew
    Song, Shumei
    Ajani, Jaffer A.
    Wang, Linghua
    [J]. NATURE MEDICINE, 2021, 27 (01) : 141 - +
  • [2] Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
    Kim, Jihyun
    Park, Charny
    Kim, Kwang H.
    Kim, Eun Hye
    Kim, Hyunki
    Woo, Jong Kyu
    Seong, Je Kyung
    Nam, Ki Taek
    Lee, Yong Chan
    Cho, Soo Young
    [J]. NPJ PRECISION ONCOLOGY, 2022, 6 (01)
  • [3] Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
    Jihyun Kim
    Charny Park
    Kwang H. Kim
    Eun Hye Kim
    Hyunki Kim
    Jong Kyu Woo
    Je Kyung Seong
    Ki Taek Nam
    Yong Chan Lee
    Soo Young Cho
    [J]. npj Precision Oncology, 6
  • [4] Single-cell profiling reveals tumor heterogeneity and cell lineage diversity in gastric cancer
    Sun, Chuwen
    [J]. GUT, 2023, 72 (SUPPL_1) : A76 - A76
  • [5] Single-cell transcriptome analysis reveals intratumoral heterogeneity in lung adenocarcinoma
    Xu, Hong
    Jiang, Lin
    Qin, Lingshan
    Shi, Ping
    Xu, Ping
    Liu, Changyu
    [J]. ENVIRONMENTAL TOXICOLOGY, 2024, 39 (03) : 1847 - 1857
  • [6] SINGLE-CELL ANALYSIS REVEALS INTRATUMORAL HETEROGENEITY IN GLIOBLASTOMA
    不详
    [J]. CANCER DISCOVERY, 2014, 4 (08) : 865 - 865
  • [7] SINGLE-CELL LINEAGE TRACING IN PRIMARY GLIOBLASTOMA REVEALS DISTINCT PROGENITOR SUBTYPES DRIVING INTRATUMORAL HETEROGENEITY
    Fazzari, Elisa
    Azizad, Daria
    Ge, Weihong
    Li, Matthew
    Tum, Andrew
    Tse, Christopher
    Patel, Kunal
    Muthukrishnan, Sree Deepthi
    Kornblum, Harley
    Nathanson, David
    Bhaduri, Aparna
    [J]. NEURO-ONCOLOGY, 2023, 25
  • [8] Diversity and intratumoral heterogeneity in human gallbladder cancer progression revealed by single-cell RNA sequencing
    Chen, Peizhan
    Wang, Yueqi
    Li, Jingquan
    Bo, Xiaobo
    Wang, Jie
    Nan, Lingxi
    Wang, Changcheng
    Ba, Qian
    Liu, Houbao
    Wang, Hui
    [J]. CLINICAL AND TRANSLATIONAL MEDICINE, 2021, 11 (06):
  • [9] Single-cell transcriptomics reveals clonal diversity and tumor heterogeneity in gastric cancer
    Koh, Vivien
    Kalpana, Ramnarayanan
    Li, Huipeng
    Nandi, Tannistha
    Tan, Angie
    Prabhakar, Shyam
    Chen, Jinmiao
    Chan, Shing Leng
    Shabbir, Asim
    So, Jimmy
    Tan, Patrick
    Yong, Wei Peng
    [J]. CANCER SCIENCE, 2018, 109 : 609 - 609
  • [10] Molecular classification and intratumoral heterogeneity of gastric adenocarcinoma
    Kuwata, Takeshi
    [J]. PATHOLOGY INTERNATIONAL, 2024, 74 (06) : 301 - 316