The study evaluated the safety and colonisation properties of four lactic acid bacteria (LAB), by determining their cell hydrophobicity and aggregation abilities. In addition, the presence of virulence and resistance genes was assayed in these probiotic candidates. Lactobacillus reuteri ZJ625, Lactobacillus reuteri VB4, Lactobacillus salivarius ZJ614 and Streptococcus salivarius NBRC13956 were tested for cell surface hydrophobicity abilities against xylene, chloroform and ethyl acetate. The isolates were also tested for auto-aggregation and co-aggregation abilities; the optical densities of cell growth were measured after 1, 2, 3 and 4 h of experimental set-up. DNA was extracted from all the four isolates and amplified using PCR with specific primers to detect virulence genes of adhesion collagen protein (ace) and aggregation substances (agg and asa); also, resistance genes of Vancomycin vanA, Vancomycin vanC1 and Vancomycin vanC2/3 were assayed in the four isolates. The isolates showed high hydrophobicity to all solvents: xylene (78–84%), chloroform (68–75%) and ethyl acetate (52–60%). High auto- and co-aggregations ranging from 60 to 70% and from 45 to 56% respectively were observed in the isolates after 4 h of incubation at 37 °C. Some of the tested isolates showed the presence of virulence and resistance genes; however, this does not indicate that these genes are unsafe because their transmission and expression abilities are unknown. Therefore, in this study, the isolates studied are considered safe for use as future probiotics, as revealed from results presented, which generally represents the scanned safety evaluations of the isolates as promising probiotics.
机构:
Agr Res Council, Anim Prod Inst, Gastrointestinal Microbiol & Biotechnol Div, ZA-0062 Irene, South Africa
Univ Ft Hare, SAMRC Microbial Water Qual Monitoring Ctr, ZA-5700 Alice, South AfricaAgr Res Council, Anim Prod Inst, Gastrointestinal Microbiol & Biotechnol Div, ZA-0062 Irene, South Africa
Dlamini, Ziyanda C.
Langa, Rashwahla L. S.
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Agr Res Council, Anim Prod Inst, Gastrointestinal Microbiol & Biotechnol Div, ZA-0062 Irene, South AfricaAgr Res Council, Anim Prod Inst, Gastrointestinal Microbiol & Biotechnol Div, ZA-0062 Irene, South Africa
Langa, Rashwahla L. S.
Aiyegoro, Olayinka A.
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Agr Res Council, Anim Prod Inst, Gastrointestinal Microbiol & Biotechnol Div, ZA-0062 Irene, South Africa
Univ Ft Hare, SAMRC Microbial Water Qual Monitoring Ctr, ZA-5700 Alice, South AfricaAgr Res Council, Anim Prod Inst, Gastrointestinal Microbiol & Biotechnol Div, ZA-0062 Irene, South Africa
Aiyegoro, Olayinka A.
Okoh, Anthony I.
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Univ Ft Hare, SAMRC Microbial Water Qual Monitoring Ctr, ZA-5700 Alice, South AfricaAgr Res Council, Anim Prod Inst, Gastrointestinal Microbiol & Biotechnol Div, ZA-0062 Irene, South Africa
机构:
Handong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South KoreaHandong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South Korea
Rwubuzizi, Ronaldo
Kim, Hamin
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Handong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South KoreaHandong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South Korea
Kim, Hamin
Holzapfel, Wilhelm Heinrich
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Handong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South KoreaHandong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South Korea
Holzapfel, Wilhelm Heinrich
Todorov, Svetoslav Dimitrov
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Handong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South Korea
Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, Lab Microbiol Alimentos,ProBacLab, BR-05508000 Sao Paulo, SP, BrazilHandong Global Univ, Dept Adv Convergence, ProBacLab, Gyeongbuk 37554, Pohang, South Korea
机构:
Univ Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania
Natl Inst Res & Dev Biol Sci, Dept Biotechnol, Bucharest, RomaniaUniv Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania
Dobre, Andreea
Sicuia, Oana
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Univ Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania
Res & Dev Inst Plant Protect, Dept Beneficial Microorganisms, Bucharest, RomaniaUniv Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania
Sicuia, Oana
Grosu, Iulian Alexandru
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Univ Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, RomaniaUniv Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania
Grosu, Iulian Alexandru
Ciobotaru, Oana
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Univ Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, RomaniaUniv Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania
Ciobotaru, Oana
Butu, Alina Elena
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Natl Inst Res & Dev Biol Sci, Dept Biotechnol, Bucharest, RomaniaUniv Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania
Butu, Alina Elena
Cornea, Calina Petruta
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Univ Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, RomaniaUniv Agron Sci & Vet Med Bucharest, Fac Biotechnol, Bucharest, Romania