FixNCut: single-cell genomics through reversible tissue fixation and dissociation

被引：0

作者：

Laura Jiménez-Gracia

Domenica Marchese

Juan C. Nieto

Ginevra Caratù

Elisa Melón-Ardanaz

Victoria Gudiño

Sara Roth

Kellie Wise

Natalie K. Ryan

Kirk B. Jensen

Xavier Hernando-Momblona

Joana P. Bernardes

Florian Tran

Laura Katharina Sievers

Stefan Schreiber

Maarten van den Berge

Tessa Kole

Petra L. van der Velde

Martijn C. Nawijn

Philip Rosenstiel

Eduard Batlle

Lisa M. Butler

Ian A. Parish

Jasmine Plummer

Ivo Gut

Azucena Salas

Holger Heyn

Luciano G. Martelotto

机构：

[1] Centro Nacional de Análisis Genómico (CNAG),Inflammatory Bowel Disease Group

[2] Universitat de Barcelona (UB),Cancer Immunology Program

[3] Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS),Sir Peter MacCallum Department of Oncology

[4] Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD),Monash University Department of Surgery

[5] Peter MacCallum Cancer Centre,Adelaide Centre for Epigenetics (ACE)

[6] The University of Melbourne,South Australian immunoGENomics Cancer Institute (SAiGENCI)

[7] Alfred Hospital,Freemasons Foundation Centre for Men’s Health

[8] University of Adelaide,Institute of Clinical Molecular Biology

[9] University of Adelaide,Department of Internal Medicine I

[10] Australian Genomics Research Facility,Department of Pulmonary Diseases

[11] University of Adelaide,Department of Pathology & Medical Biology

[12] South Australian Health and Medical Research Institute,undefined

[13] Institute for Research in Biomedicine (IRB Barcelona),undefined

[14] The Barcelona Institute of Science and Technology,undefined

[15] Centro de Investigación Biomédica en Red de Cáncer (CIBERONC),undefined

[16] Kiel University,undefined

[17] University Hospital Schleswig-Holstein (UKSH),undefined

[18] University of Groningen,undefined

[19] University Medical Center Groningen,undefined

[20] Groningen Research Institute for Asthma and COPD,undefined

[21] University of Groningen,undefined

[22] University Medical Center Groningen,undefined

[23] University of Groningen,undefined

[24] University Medical Center Groningen,undefined

[25] ICREA,undefined

[26] St Jude Children’s Research Hospital,undefined

[27] Omniscope,undefined

来源：

Genome Biology | / 25卷

关键词：

Single-cell genomics; RNA sequencing; Sample fixation; Tissue dissociation; Cellular stress;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced during specimen handling. We present FixNCut, a methodology for the reversible fixation of tissue followed by dissociation that overcomes current limitations. We applied FixNCut to human and mouse tissues to demonstrate the preservation of RNA integrity, sequencing library complexity, and cellular composition, while diminishing stress-related artifacts. Besides single-cell RNA sequencing, FixNCut is compatible with multiple single-cell and spatial technologies, making it a versatile tool for robust and flexible study designs.

引用

共 50 条

[1] FixNCut: single-cell genomics through reversible tissue fixation and dissociation
Jimenez-Gracia, Laura
Marchese, Domenica
Nieto, Juan C.
Caratu, Ginevra
Melon-Ardanaz, Elisa
Gudino, Victoria
Roth, Sara
Wise, Kellie
Ryan, Natalie K.
Jensen, Kirk B.
Hernando-Momblona, Xavier
Bernardes, Joana P.
Tran, Florian
Sievers, Laura Katharina
Schreiber, Stefan
van den Berge, Maarten
Kole, Tessa
van der Velde, Petra L.
Nawijn, Martijn C.
Rosenstiel, Philip
Batlle, Eduard
Butler, Lisa M.
Parish, Ian A.
Plummer, Jasmine
Gut, Ivo
Salas, Azucena
Heyn, Holger
Martelotto, Luciano G.
GENOME BIOLOGY, 2024, 25 (01)
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