Development and characterization of 148 SNP markers in the caribbean symmetrical brain coral Pseudodiploria strigosa

As coral reefs decline, restoring lost coral populations has been hampered due to the paucity of genetic information available for many coral species. In the Caribbean, the symmetrical brain coral Pseudodiploria strigosa, a prominent framework builder of the reef, has experienced an accelerated decline due to stony coral tissue loss disease (SCTLD). Colonies of P. strigosa gene-banked in response to the threat of SCTLD in Florida, USA, were sampled to develop 148 novel single nucleotide polymorphism (SNP) markers using genotyping-by-sequencing (GBS). The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.008 to 0.888 and 0.018 to 0.500, respectively. Deviations from Hardy–Weinberg equilibrium within populations, measured by the inbreeding coefficient index (Fis), ranged from − 0.799 to 0.923. In total, 96 SNPs were found to deviate significantly from Hardy–Weinberg (p < 0.05). These SNPs can be used for genetic population analysis to assist management and restoration of P. strigosa.