Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish Holstein-Friesian cattle

被引：0
|
作者：
Urszula Czarnik
Tadeusz Zabolewicz
Janusz Strychalski
Grzegorz Grzybowski
Marcin Bogusz
Krzysztof Walawski
机构：
[1] University of Warmia and Mazury,Department of Animal Genetics
[2] Polish Academy of Sciences,Institute of Animal Genetics and Breeding
来源：
Journal of Applied Genetics | 2007年 / 48卷
关键词：
Polish Holstein-Friesian cattle; prion; PRNP; polymorphism; haplotypes;
D O I：
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中图分类号：
学科分类号：
摘要：
The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3’ untranslated region (14 bp). DNA was isolated from blood of 234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004. No statistically significant differences were found in the frequency of genotypes and alleles between cows and breeding bulls in the 3 analysed polymorphic sites within thePRNP gene. Only 3 haplotypes were identified in sires and 4 haplotypes in cows.
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页码：69 / 71
页数：2
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