Potentilla chinensis aqueous extract attenuates cyclophosphamide-induced hemorrhagic cystitis in rat model

被引：0
作者：
Kajetan Juszczak
Jan Adamowicz
Łukasz Zapała
Tomasz Kluz
Przemysław Adamczyk
Artur Wdowiak
Iwona Bojar
Marcin Misiek
Magdalena Emilia Grzybowska
Klaudia Stangel-Wójcikiewicz
Ewa Poleszak
Marta Pokrywczyńska
Tomasz Drewa
Andrzej Wróbel
机构：
[1] Nicolaus Copernicus University,Department of Urology and Andrology, Collegium Medicum
[2] Nicolaus Copernicus University,Department of Regenerative Medicine, Collegium Medicum
[3] Medical University of Warsaw,Clinic of General, Oncological and Functional Urology
[4] Medical College of Rzeszow University,Department of Gynecology and Obstetrics, Institute of Medical Sciences
[5] Nicolaus Copernicus Hospital,Department of General and Oncological Urology
[6] Medical University of Lublin,Chair of Obstetrics and Gynecology, Faculty of Health Sciences
[7] Institute of Rural Health in Lublin,Department of Women’s Health
[8] Holy Cross Cancer Center,Department of Gynecologic Oncology
[9] University of Gdańsk,Department of GynecologyGynecological Oncology and Gynecological Endocrinology, Medical
[10] Jagiellonian University Medical College,Department of Gynecology and Oncology
[11] Medical University of Lublin,Chair and Department of Applied and Social Pharmacy, Laboratory of Preclinical Testing
[12] Medical University of Lublin,Second Department of Gynaecology
来源：
Scientific Reports | / 12卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Cyclophosphamide (CYP) damages all mucosal defence lines and induces hemorrhagic cystitis (HC) leading to detrusor overactivity. Patients who undergo combined chemio-radiotherapy are at higher risk of HC. Potentilla chinensis extract (PCE) prevent oxidative stress-dependent diseases. Thus, the aim of the study was to investigate the effect of PCE on urinary bladder function in CYP-induced HC in preclinical study. 60 rats were divided into 4 groups, as follows: I—control, II—rats with CYP-induced HC, III—rats received PCE in dose of 500 mg/kg, and IV—rats with CYP-induced HC which received PCE in dose of 500 mg/kg. PCE or vehicle were administered orally for 14 days. The cystometry was performed 3 days after the last dose of the PCE. Next, urothelium thickness and oedema measurement and biochemical analyses were performed. Cyclophosphamide induced hemorrhagic cystitis. PCE had no influence on the urinary bladder function and micturition cycles in normal rats. PCE diminished the severity of CYP-induced hemorrhagic cystitis. In the urothelium the cyclophosphamide induced the elevation of CGRP, TNF-α, IL-6, IL-1β, OTC3, NIT, and MAL. Also, the level of T-H protein, HB-EGF, and ZO1 was decreased. Moreover, the level of ROCK1 and VAChT in detrusor muscle increased. cyclophosphamide caused an increased concentration of BDNF and NGF in the urine. In turn, PCE in cyclophosphamide-induced hemorrhagic cystitis caused a reversal of the described biochemical changes within urothelium, detrusor muscle and urine. PCE attenuates detrusor overactivity. In conclusion, our results revealed that PCE attenuates detrusor overactivity in case of cyclophosphamide-induced hemorrhagic cystitis. The potential properties of PCE appear to be important in terms of preventing of oxidative stress-dependent dysfunction of urinary bladder. PCE may become a potential supportive treatment in patient to whom cyclophosphamide-based chemotherapy is used.
引用
收藏
相关论文
共 263 条
  • [1] Ritchey M(2009)Late effects on the urinary bladder in patients treated for cancer in childhood: A report from the Children's Oncology Group Pediatr. Blood Cancer. 52 439-446
  • [2] Ferrer F(1979)Cyclophosphamide cystitis: Identification of acrolein as a causative agent Biochem. Pharmacol. 28 2045-2049
  • [3] Shearer P(1989)Urological complications of cyclophosphamide J. Urol. 141 1063-1069
  • [4] Spunt SL(1988)Efficacy of mesna in preventing further cyclophosphamide-induced hemorrhagic cystitis Med. Pediatr. Oncol. 16 372-374
  • [5] Cox PJ(2019)Uroprotective effect of ambroxol in cyclophosphamide-induced cystitis in mice Int. Urol. Nephrol. 51 803-810
  • [6] Levine LA(2018)Cytoprotective effects of glycyrrhetinic acid liposome against cyclophosphamide-induced cystitis through inhibiting inflammatory stress Int. Immunopharmacol. 54 139-144
  • [7] Richie JP(2009)Inflammation and chronic oxidative stress in radiation-induced late normal tissue injury: Therapeutic implications Curr. Med. Chem. 16 130-143
  • [8] Luce JK(2015)Potentilla and rubus medicinal plants: Potential non-camellia tea resources Med. Plants. 1 373-430
  • [9] Simons JA(2020)The influence of Biomed. Pharmacother. 132 515-521
  • [10] Barut EN(2011) aqueous extract on urinary bladder function in retinyl acetate-induced detrusor overactivity in rats J. Ethnopharmacol. 135 683-690
12345678910