Twenty-four polymorphic microsatellite markers from spotted barbell (Hemibarbus maculates)

Spotted barbell (Hemibarbus maculates) is an economically important freshwater fish which is widely distributed throughout China’s river systems. We reported the isolation and characterization of 24 polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA)15 probe. The variability of these microsatellites were tested on 30 individuals. The average allele number was 10.5 per locus, ranging from 3 to 18. The observed heterozygosity was from 0.0000 to 1.0000 and the expected heterozygosity was from 0.6226 to 0.9379. 6 of the 24 microsatellites did not conform to Hardy–Weinberg equilibrium. These microsatellites can be used to study economic traits of QTL position, population genetic diversity and the construction of the genetic mapping for Hemibarbus maculates in the future.