Accurate identification of single-nucleotide variants in whole-genome-amplified single cells

被引：0

作者：

Dong X. ^{[1
]}

Zhang L. ^{[1
]}

Milholland B. ^{[1
]}

Lee M. ^{[1
]}

Maslov A.Y. ^{[1
]}

Wang T. ^{[2
]}

Vijg J. ^{[1
,3
]}

机构：

[1] Department of Genetics, Albert Einstein College of Medicine, Bronx, NY

[2] Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, NY

[3] Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, NY

来源：

Nature Methods | 2017年 / 14卷 / 5期

关键词：

D O I：

10.1038/nmeth.4227

中图分类号：

学科分类号：

摘要：

Mutation analysis in single-cell genomes is prone to artifacts associated with cell lysis and whole-genome amplification. Here we addressed these issues by developing single-cell multiple displacement amplification (SCMDA) and a general-purpose single-cell-variant caller, SCcaller (https://github.com/biosinodx/SCcaller/). By comparing SCMDA-amplified single cells with unamplified clones from the same population, we validated the procedure as a firm foundation for standardized somatic-mutation analysis in single-cell genomics. © 2017 Nature America, Inc. part of Springer Nature. All rights reserved.

引用

页码：491 / 493

页数：2

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