Utility of the FLP-FRT recombination system for genetic manipulation of rice

被引：5
|
作者：
Parthiban Radhakrishnan
Vibha Srivastava
机构：
[1] University of Arkansas,Department of Crop, Soil and Environmental Sciences
[2] University of Arkansas,Department of Horticulture
来源：
Plant Cell Reports | 2005年 / 23卷
关键词：
FLP-; Cre-; Site-specific recombination; Rice transformation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To develop an FLP-FRT recombination system- (derived from 2 μ plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the β-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system (derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice.
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页码：721 / 726
页数：5
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