Development and characterization of twelve novel polymorphic microsatellite loci in the Percocypris pingi

Percocypris pingi is an endemic subspecies of Percocypris mainly distributed in upper Yangtze river (Jinsha river) and its branch river. This species has been listed as a near threatened species in IUCN Red List. Twelve microsatellite markers were developed and the allele number of these polymorphic markers ranged from 2 to 5. Observed and expected heterozygosity in the population ranged from 0.381 to 0.952 and from 0.517 to 0.787, respectively. No significant linkage disequilibrium was detected among the loci. No loci significantly (P < 0.004) deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These microsatellite markers will provide helpful tools not only for genetic analyses of wild P. pingi but also for possible re-stocking management in the long turn.