Simulation Model for Three-Channel Luminescent Images of Cancer Cell Populations

被引：0
|
作者：
E. V. Lisitsa
M. M. Yatskou
V. V. Apanasovich
T. V. Apanasovich
M. M. Shytsik
机构：
[1] Belorussian State University,
来源：
Journal of Applied Spectroscopy | 2015年 / 81卷
关键词：
luminescence microscopy; cell; simulation model; segmentation algorithm;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have developed a simulation model for three-channel luminescent images of cancer cells. We do a comparative analysis of two methods for segmentation of objects within three-channel luminescent images, employed in the CellProfiler and CellDataMiner software packages, using simulated and experimental data as examples. We have studied the functional efficiency of the segmentation algorithms under high photoelectron multiplier noise conditions. Our simulation model allows us to simplify selection of the algorithm for analysis of the data during biophysical studies, with the aim of automating procedures for processing luminescent images.
引用
收藏
页码：996 / 1003
页数：7
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