Ultra-high-throughput sequencing of the immune receptor repertoire from millions of lymphocytes

被引：0

作者：

Jonathan R McDaniel

Brandon J DeKosky

Hidetaka Tanno

Andrew D Ellington

George Georgiou

机构：

[1] University of Texas at Austin,Department of Chemical Engineering

[2] Institute for Cellular and Molecular Biology,Department of Molecular Biosciences

[3] University of Texas at Austin,undefined

[4] Center for Systems and Synthetic Biology,undefined

[5] University of Texas at Austin,undefined

[6] University of Texas at Austin,undefined

[7] Present address: Vaccine Research Center,undefined

[8] National Institute of Allergy and Infectious Diseases,undefined

[9] National Institutes of Health,undefined

[10] Bethesda,undefined

[11] Maryland,undefined

[12] USA.,undefined

来源：

Nature Protocols | 2016年 / 11卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This protocol uses a microfluidic flow-focusing device to encapsulate single cells, enabling high-throughput sequencing of the paired immune receptor repertoire from millions of lymphocytes at the single-cell level.

引用

页码：429 / 442

页数：13

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