Identification of a novel bluetongue virus 1-specific B-cell epitope using a monoclonal antibody against the VP2 protein

被引：0
|
作者：
P. Wei
E. C. Sun
N. H. Liu
T. Yang
Q. Y. Xu
J. Zhao
Y. L. Qin
Y. F. Feng
J. P. Li
W. S. Wang
C. Y. Zhang
D. L. Wu
机构：
[1] Chinese Academy of Agricultural Sciences,The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute
[2] Graduate School of Chinese Academy of Agricultural Sciences,College of Animal Medicine
[3] Northeast Agricultural University,College of Animal Medicine
[4] HuNan Agricultural University,undefined
来源：
Archives of Virology | 2013年 / 158卷
关键词：
BHK21 Cell; Indirect ELISA; BTV1 Isolate; Linear Peptide Epitope;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Bluetongue virus (BTV) VP2 is an important antigenic protein that can be used for the differential diagnosis of different BTV serotypes. Here, we generated a serotype-specific monoclonal antibody (mab) against BTV1. A series of peptides synthesized based on the amino acid sequence of BTV1 VP2 were screened to define 115AQPLKVGL122 as the minimal linear peptide epitope recognized by mab 4B6. Using an immunofluorescence assay (IFA), we found that mab 4B6 reacted strongly with BTV1, but did not react with other BTV serotypes (BTV2-24). The 4B6 will serve as a novel reagent in the development of diagnostic tests for BTV1 infection.
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页码：1099 / 1104
页数：5
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