TLC-Densitometric Analysis of p-Sitosterol in Pumpkin Seed Oil

被引：0
|
作者：
Małgorzata Starek
Jan Krzek
机构：
[1] Jagiellonian University,Collegium Medicum, Department of Inorganic and Analytical Chemistry
来源：
JPC – Journal of Planar Chromatography – Modern TLC | 2007年 / 20卷
关键词：
Pumpkin seed oil; TLC-densitometry; Sitosterols Sylwia Michnik;
D O I：
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中图分类号：
学科分类号：
摘要：
A densitometric thin-layer chromatographic method has been established for quantitative determination of P-sitosterol in pumpkin seed oil (Cucurbita pepo L.). Chromatography was performed on silica gel 60 F254 TLC plates, with toluene-ethyl acetate-glacial acetic acid 15:4:1 (v/v) as mobile phase. Densitometric quantitation was performed at 525 nm. The method was validated by determination of linearity (15-750 μg mL-1), precision (RSD = 2.36%), and limits of detection (LOD = 0.65 μg per band) and quantification (LOQ = 1.99 μg per band). Average recovery from the pharmaceutical preparation was 96.15%. The method enables simple, rapid, and precise quantitative determination of P-sitosterol in pharmaceutical preparations and can be used for routine quality control.
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页码：327 / 330
页数：3
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