Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

被引：0

作者：

Camila C. B. Brito

Fernando V. Maluf

Gustavo M. A. de Lima

Rafael V. C. Guido

Marcelo S. Castilho

机构：

[1] Universidade Estadual de Feira de Santana,Programa de pós

[2] University of Sao Paulo,graduação em Biotecnologia

[3] Universidade Federal da Bahia,Sao Carlos Institute of Physics

[4] Lund University,Faculdade de Farmácia

来源：

Molecular Biotechnology | 2018年 / 60卷

关键词：

Superoxide dismutase; Heterologous expression; Kinetic assay;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).

引用

页码：595 / 600

页数：5

共 50 条

[1] Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis
Brito, Camila C. B.
Maluf, Fernando V.
de Lima, Gustavo M. A.
Guido, Rafael V. C.
Castilho, Marcelo S.
MOLECULAR BIOTECHNOLOGY, 2018, 60 (08) : 595 - 600
[2] Molecular cloning and biochemical characterization of iron superoxide dismutase from the rodent malaria parasite Plasmodium vinckei
Prakash, Kirtika
Goyal, Manish
Soni, Awakash
Siddiqui, Arif Jamal
Bhardwaj, Jyoti
Puri, Sunil K.
PARASITOLOGY INTERNATIONAL, 2014, 63 (06) : 817 - 825
[3] Cloning, characterization and overexpression of two iron superoxide dismutase cDNAs from Leishmania chagasi:: role in pathogenesis
Paramchuk, WJ
Ismail, SO
Bhatia, A
Gedamu, L
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 90 (01) : 203 - 221
[4] Molecular cloning and characterization of two iron superoxide dismutase cDNAs from Trypanosoma cruzi
Ismail, SO
Paramchuk, W
Skeiky, YAW
Reed, SG
Bhatia, A
Gedamu, L
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 86 (02) : 187 - 197
[5] Molecular cloning and biochemical characterization of a Cu,Zn-superoxide dismutase from Scedosporium apiospermum
Lima, Osana C.
Larcher, Gerald
Vandeputte, Patrick
Lebouil, Anne
Chabasse, Dominique
Simoneau, Philippe
Bouchara, Jean-Philippe
MICROBES AND INFECTION, 2007, 9 (05) : 558 - 565
[6] MOLECULAR-CLONING, CHARACTERIZATION, AND EXPRESSION IN ESCHERICHIA-COLI OF IRON SUPEROXIDE-DISMUTASE CDNA FROM LEISHMANIA-DONOVANI CHAGASI
ISMAIL, SO
SKEIKY, YAW
BHATIA, A
OMARAOPYENE, LA
GEDAMU, L
INFECTION AND IMMUNITY, 1994, 62 (02) : 657 - 664
[7] Purification, characterization, and molecular cloning of a thermostable superoxide dismutase from Thermoascus aurantiacus
E, Shijin
Guo, Fangxian
Liu, Shouan
Chen, Jing
Wang, Yanjun
Li, Duochuan
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2007, 71 (04) : 1090 - 1093
[8] Cloning and molecular characterization of the cDNA encoding histone H1 from Leishmania braziliensis
Martínez, E
Thomas, MC
Alonso, V
Carmelo, E
González, AC
Del Castillo, A
Valladares, B
JOURNAL OF PARASITOLOGY, 2002, 88 (01) : 199 - 203
[9] Cloning and molecular characterization of Cu,Zn superoxide dismutase from Actinobacillus pleuropneumoniae
Langford, PR
Loynds, BM
Kroll, JS
INFECTION AND IMMUNITY, 1996, 64 (12) : 5035 - 5041
[10] Cloning, overexpression, purification, and characterization of a new iron superoxide dismutase from Jatropha curcas
Chao Ou-yang
Cai, Feng
Gao, Shun
Niu, Bei
Wang, Shenghua
Chen, Fang
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2012, 59 (05) : 338 - 345

← 1 2 3 4 5 →