High-throughput DNA extraction from forest trees

被引：4
|
作者：
Shepherd M. [1 ,2 ]
Cross M. [1 ,2 ]
Stokoe R.L. [1 ,2 ]
Scott L.J. [1 ,2 ]
Jones M.E. [2 ]
机构：
[1] Coop. Res. Ctr. Sustainable Prod. F., Ctr. for Plant Conservation Genetics, Southern Cross University, Lismore, NSW 2480, Military Rd
[2] Ctr. for Plant Conservation Genetics, Southern Cross University, Lismore, NSW 2480, Military Rd
来源：
Plant Molecular Biology Reporter | 2002年 / 20卷 / 4期
关键词：
AFLP; Araucaria; Eucalyptus; Microsatellite; Pinus;
D O I：
10.1007/BF02772134
中图分类号：
学科分类号：
摘要：
It is difficult to extract pure high-quality DNA from trees, which may not be amenable to advances in extraction methods suitable for other plants. A new commercial high-throughput DNA extraction system, using a silica binding matrix for purification and a multisample mixer mill for tissue disruption, was evaluated for its suitability with Eucalyptus spp., Pinus spp., and Araucaria cunninghamii (hoop pine). DNA suitable for a range of molecular biology applications was successfully extracted from all genera. The method was highly reliable when tested in more than 500 preparations and could be adapted to different tree species with relatively minor modifications.
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收藏
页码：425a / 425f
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