Determination of the Enantiomeric Purity of l-Carnitine and Acetyl-l-Carnitine by Chiral Liquid Chromatography

被引：1
|
作者：
Meiling Qi
Peng Wang
Yujing Sun
Jianjun Yang
机构：
[1] Beijing Institute of Technology,Department of Chemistry, School of Chemical Engineering and Materials Science
[2] Shenyang Pharmtech Institute of Pharmaceuticals,undefined
[3] Hebei Hengli Pharmaceutical Group,undefined
来源：
Chromatographia | 2004年 / 59卷
关键词：
Column liquid chromatography Enantiomeric purity ; -Carnitine Acetyl-; -carnitine;
D O I：
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中图分类号：
学科分类号：
摘要：
A chiral liquid chromatographic method for determination of the enantiomeric purity of both l-carnitine and acetyl-l-carnitine is described. Separation of the enantiomers of dl-carnitine and acetyl-dl-carnitine was achieved on a commercial chiral column (Chiralcel OD-R) after derivatization with (alpha-bromo)methyl phenyl ketone. Introduction of this lipophilic UV chromophoric group to the carnitine and acetylcarnitine molecules improved their retention, resolution, and UV detection. The mobile phase was 74:26 (v/v) 0.5 mol L-1 sodium perchlorate–acetonitrile, pH 3.8, and the flow rate was 0.4 mL min-1. Detection was performed at 235 nm. The method is selective and reliable for determination of the enantiomeric purity of bulk drug substances l-carnitine and acetyl-l-carnitine.
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页码：247 / 250
页数：3
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