Relationship between GSTM1 and CYP1A1 polymorphisms in colorectal carcinoma Egyptian patients

Colorectal cancer (CRC) is a multifactorial disease with dietary, lifestyle, and environmental exposures on one hand and genetic predispositions on the other hand. In this study, we examined the hypothesis of whether genetic polymorphisms in glutathione S-transferase M1 (GSTM1) and cytochrome P450 1A1 (CYP1A1) are related to the risk of developing CRC in Egyptian patients. The association of polymorphisms of CYP1A1 and GSTM1 with CRC was investigated in 40 patients (group I) and 40 controls (group II) using multiplex PCR for GSTM1 and PCR-RFLP for CYP1A1 polymorphism detection. The GSTM1 null genotype was more frequent in group I (45%) than in group II (35%), but this difference was not statistically significant [odds ratio (OR), 0.658 and the 95% confidence interval (CI), 0.217-1.997]. The relationship of GSTM1 deficiency and CRC risk was performed between both sexes; p = 0.213, tumor site p = 0. 232, and pathological type p = 0. 2, but none of them was statistically significant. The CYP1A1 variant genotype was more frequent in group II (25%) than in group I (10%), but this difference was not statistically significant (OR, 0.333 with the 95% CI, 0.078-1.416). The relationship of CYP1A1 and CRC risk was also performed between both sexes, p = 0.30, tumor site p = 0.329, and pathological type p = 0.16, but none of them was statistically significant. The results of this study suggest that neither the GSTM1 nor the CYP1A1 is associated with the risk of development of colorectal cancer in Egyptian patients. © 2011 Springer-Verlag London Limited.